Composite

Part:BBa_K4350142:Design

Designed by: Trevor Fox   Group: iGEM22_UBC-Okanagan   (2022-09-30)


Neonothopanus nambi Hispidin-3-hydroxylase (nnH3H) TU (PlaqIQ, TrrnB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1391
    Illegal BsaI site found at 1569


Design Notes

PlaqIQ was chosen as it is a strongly expressed synthetic constitutive promoter for E. coli.

This transcriptional unit is designed for expression in E. coli. as a proof-of-concept for prokaryotic expression of fungal luciferase (nnLuz) (BBa_K4350814).


Source

The sequence for this enzyme was retrieved from https://www.pnas.org/doi/10.1073/pnas.1803615115. Promoter and terminator from registry.

References